Includes bibliographical references and index.
|Statement||edited by S. Gupta ... [et al.].|
|Series||Falk symposium ;, 126|
|LC Classifications||RD546 .F34 2001|
|The Physical Object|
|Pagination||ix, 173 p. :|
|Number of Pages||173|
|LC Control Number||2003277433|
Sherilyn Gordon Burroughs, R. Mark Ghobrial, in Transplantation of the Liver (Third Edition), General Overview and Indications. Hepatocyte transplantation is a novel, less invasive, alternative therapy to solid organ liver transplant that provides synthetic and hepatocyte function for patients with acute liver failure and congenital hepatic-dominant metabolic disturbances. Hepatocyte transplantation has many potential applications. Therapeutic genes can be transferred into cultured hepatocytes, and the phenotypically modified cells can then be transplanted for ex vivo gene therapy. Such gene transfer could be used to replace a missing gene product, to prevent immune rejection, or to give the cells a proliferative. Buy Hepatocyte Transplantation: Methods and Protocols (Methods in Molecular Biology) on FREE SHIPPING on qualified orders. A potential alternative to liver transplantation is allogeneic hepatocyte transplantation. Over the last two decades, hepatocyte transplantation has made the transition from bench to bedside.
In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal. ISBN: OCLC Number: Description: xiv, pages: illustrations (some color) ; 25 cm. Contents: Historical review of hepatocyte transplantation: the road from whole liver transplantation to hepatocyte transplantation / Michio Mito and Masayuki Sawa --Preparation of hepatocytes / Shinichi Kasai and Michio Mito --Hepatocyte cryopreservation / B.J. Fuller and W. Hepatocyte Transplantation: Methods and Protocols guides readers through laboratory protocols for the generation of humanized livers for the assessment of biological actions in vivo and techniques to monitor cell engraftment after cell transplantation in vivo are described and procedures for computational analyses of hepatocyte transplantation. In recent years there has been an increasing need for transplantation, but the number of donor livers available has increased only slightly, despite intensive public relations activities. New concepts in the field of transplantation, for instance the transplantation of living donor organs or Price: $
Hepatocyte Transplantation: The grafting of hepatocytes into the liver to perform defective metabolic, immune, endocrine, and homeostatic function. 80+ transplants have been performed as of late Using this transplantation model, we determined that 1) a mean of 21% of splenically injected hepatocytes engraft in liver parenchyma; 2) a mean of % of splenically injected hepatocytes (or one-third of engrafted cells) can give rise to proliferating hepatocyte foci; 3) transplanted cells in proliferating foci display an initial cell. These systems have been shown to be supportive for hepatocyte culture and have been used as scaffolds for hepatocyte transplantation. For example, a composite PLLA/PLGA scaffold was demonstrated to support long-term engraftment of hepatocytes on mesenteric transplantation in a rodent injury model (Mooney et al., ). Human hepatocyte transplantation (HT) has been shown to be of value in managing liver‐based metabolic disorders; however, the cell function starts deteriorating after 1 year. Human HT in alginate‐embedded microbeads holds promise in the management of acute liver failure without the use of immunosuppression.